Table 2 DNA glycosylase activities of the mutants.

Mutants	Substrate dsDNA	k (min⁻¹)^*	Relative activity (%)
Y68F (control)	24 bp	0.062 ± 0.003	100
	500 bp	0.038 ± 0.007	100
	3000 bp	0.052 ± 0.006	100
Y68F R70D	24 bp	0.079 ± 0.004	127
	500 bp	0.029 ± 0.008	76
	3000 bp	0.010 ± 0.001	19
Y68F D71R	24 bp	0.046 ± 0.003	74
	500 bp	0.013 ± 0.004	34
	3000 bp	0.010 ± 0.002	19
Y68F R70D D71R	24 bp	0.065 ± 0.005	105
	500 bp	0.027 ± 0.007	71
	3000 bp	0.021 ± 0.003	40
Y68F R26A	24 bp	0.040 ± 0.001	65
	500 bp	0.021 ± 0.006	55
	3000 bp	0.014 ± 0.002	27

^*The enzymatic rate constants and their standard errors were obtained from a single-exponential fit to the data from three independent measurements.

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