Figure 1 | Scientific Reports

Figure 1

From: N-linked glycosylation at Asn152 on CD147 affects protein folding and stability: promoting tumour metastasis in hepatocellular carcinoma

Figure 1

N-glycans at Asn152 are required for CD147 function.

(a) Model of the CD147 N-glycosylation site. The positions of each mutation in CD147 are illustrated. (b) Localization of CD147-EGFP. K7721 cells stably expressing wild-type or mutant CD147-EGFP (green) were immunostained using an ER-Tracker (red) and DAPI (blue) and observed using confocal laser scanning microscopy. Scale bars, 20 μm. (c) Expression of the CD147-EGFP. Cell lysates of K7721 cells stably expressing WT and each mutant were analysed by Western blotting using an anti-EGFP antibody. (d,e) Invasive potential of each cell line. Representative phase contrast image analysis and a quantitative analysis of the transwell invasive potential assay in each cell line. Values indicate the mean ± standard error of the mean (SEM) of three independent experiments. **P < 0.01. (f) Migration ability of each cell line. Representative phase contrast image analysis for the wound healing assay in each cell line. (g) Proliferation of each cell line. An in vitro cell counting kit (CCK)-8 assay was used to examine cell growth based on absorbance at 450 nm at the indicated time.

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