Figure 5

Kidneys from Olfr1393 KO mice have altered Sglt1 distribution.

Kidneys from WT and KO mice were perfused and stained for Sglt2 (A) and Sglt1 (C). Representative images from both male and female littermates are shown, and in all cases, Sglt2 staining was unchanged, and Sglt1 luminal staining was reduced in Olfr1393 KO mice. For Sglt1, the arrows indicate regions of intracellular, punctate-like accumulation. The scale bar = 20 μM. (B,D) To quantify the luminal distribution of Sglt1 and Sglt2, the mean fluorescence intensity of the luminal and total tubule stain was measured for each LTL + tubule expressing Sglt. The ratio of the luminal to total stain was calculated. In (B) WT: 0.59 ± 0.008 (n = 3 kidneys [1 male, 2 female]), KO: 0.61 ± 0.02 (n = 5 kidneys [4 male, 1 female]). In (D) WT: 0.32 ± 0.02 (n = 4 kidneys [2 male, 2 female]), KO: 0.25 ± 0.01 (n = 6 kidneys [4 male, 2 female]). Data represent mean ± SEM and * indicates p ≤ 0.05 by the student t-test.