Figure 7
Proliferation rate and motility phenotypes of human breast normal epithelium (HME1) and metastatic breast adenocarcinoma (MDA-MB-231) cells under mono- and co-culture conditions.
Compared with the BE cells, different behavior is observed. While proliferation rate (A) was slightly, but not significantly increased in the normal cells, the cancer cells proliferated significantly faster in co-culture with HME1 cells than in mono-culture (Table 2). All three motility parameters – distance, speed, and directionality – were altered significantly in both cell types under co-culture conditions (B, C, D, Supplementary Tables S4 and S5). While the average distance was slightly decreased in both cell types (B), differential response was observed in speed (C) and directionality (D). The speed of movement was decreased in HME1 cells, but increased in MDA-MB-231 cells. Directionality of HME1 cells decreased in co-culture as compared to a slight increase in MDA-MB-231 cells. Stars indicate statistical significance at p = 0.05.
