Figure 4 | Scientific Reports

Figure 4

From: Asperosaponin VI promotes bone marrow stromal cell osteogenic differentiation through the PI3K/AKT signaling pathway in an osteoporosis model

Figure 4

The mineralization of OVX rBMSCs.

(A) OVX rBMSCs were seeded in 24-well plates at a cell density of 5 × 103 and were treated with ASA VI (10−5 M) and/or LY294002 (50 μM); after 21 days, the formation of mineralized nodules was measured by alizarin red S staining. (A) After imaging the nodules, 10% cetylpyridinium chloride was used to dissolve the nodules, and mineralization was quantified using a Bio-Rad microplate reader. (B) The data are represented as the mean ± SD. **P < 0.01 vs the controls; #P < 0.05 and ##P < 0.01 vs ASA VI 10−5 M.

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