Figure 5 | Scientific Reports

Figure 5

From: Asperosaponin VI promotes bone marrow stromal cell osteogenic differentiation through the PI3K/AKT signaling pathway in an osteoporosis model

Figure 5

Cultured cells were treated with ASA VI 10−5 M for 48 hours with or without LY294002 (50 μM).

Total RNA was then extracted for quantitative PCR to assess the mRNA levels of osteogenesis-related genes, including (A) ALP, (B) OCN, (C) COL 1, and (D) RUNX2. The data are represented as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 vs the controls; #P < 0.05, ##P < 0.01, and ###P < 0.01 vs ASA VI 10−5 M.

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