Figure 1 | Scientific Reports

Figure 1

From: Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression – implications for atherosclerosis research

Figure 1

BMDMs and PEMs have a different inflammatory response to oxLDL stimulation.

(a–d) Cholesterol or MCP-1 content (% of control (0 μg/mL oxLDL)) in BMDMs (a,c) and PEMs (b,d) after stimulation with 0–50 μg/mL oxLDL for 24 hours (each graph represents 3 separate experiments (n = 3–4 wells per experiment)). *p < 0.05, **p < 0.01, ****p < 0.0001, as determined by 1-way ANOVA with Dunnets post-test. Each graph represents one representative experiment (triplicate analyses of BMDMs from one mouse or PEMs from a pool 4–5 of mice). Experiments were triplicated with similar results (i.e. n = 3 mice for BMDMs and 3 pools of 4–5 mice for PEMs). (e–g) mRNA expression (2−ΔCT) of monocyte chemoattractant protein 1 (MCP-1) (e), chemokine (C-X-C motif) ligand 1 (Cxcl 1) (f) or osteopontin (OPN) (g) in BMDMs or PEMs after incubation with 0 μg/mL oxLDL (macrophage, Mø) or 25 μg/mL oxLDL (foam cells, Fc) for 24 hours (n = 3 mice for BMDMs; n = 3 pools of mice for PEMs). *p < 0.5, **p < 0.01, as determined by 2-way ANOVA.

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