Table 1 mRNA expression of genes related to M1 and M2 differentiation or lipid metabolism.

From: Bone marrow-derived and peritoneal macrophages have different inflammatory response to oxLDL and M1/M2 marker expression – implications for atherosclerosis research

Gene of interest

Macrophage (Mø)

Foam cells (Fc)

Foam cell rel. to macrophage

PEM rel. to BMDM

PEM rel. to BMDM

BMDM

PEM

M2-associated markers

 Arg1

8757.40**

58.69

0.85

0.01

 Chi3l1

23.47

3.94

2.45

0.41

 Socs2

42.48****

45.39****

0.74

0.79

 CCL17

8691.35****

5058.71****

1.08

0.63

 CD206

0.06****

0.09****

1.11

1.64

 MGL

0.26*

0.31

0.70

0.81

 Retnla

N.D.

N.D.

N.D.

N.D.

 IL10

N.D.

N.D.

N.D.

N.D.

 MGL2

N.D.

N.D.

N.D.

N.D.

 IL4

N.D.

N.D.

N.D.

N.D.

 CD163

N.D.

N.D.

N.D.

N.D.

M1-associated markers

 IL12a

1384.57**

410.32*

1.08

0.32

 IL6

381.66*

46.07

2.15

0.26

 Ptgs2

46.15***

14.21**

0.58

0.18

 TNF

4.03**

2.67

0.61

0.40*

 CCL22

163.53

575.32

0.28

0.98

 CD11c

5.93

4.19

0.79

0.56

 Ifnß1

15.80

48.49

0.62

1.91

 Nos2

0.80

29.82

0.03

1.14

 CXCL10

0.18*

1.60

0.04***

0.33***

 Ifnɣ

N.D.

N.D.

N.D.

N.D.

Lipid metabolism

 MSR1

2.40*

1.44

0.71

0.42*

 LOX1

2774.26****

980.00****

1.22

0.43

 SREBP2

2.85**

1.20

0.91

0.38**

 ACSL1

4.01

1.47

0.95

0.35

 Abcg1

0.17***

0.56

1.58

5.26***

 DGAT2

0.99

0.93

0.32*

0.31

 SRB1

1.11

0.73

0.61

0.40**

 CD36

1.14

1.07

3.77

3.52

 SREBP1

1.57

0.86

2.03*

1.11

 Abca1

1.13

0.75

1.94

1.29

 DGAT1

1.09

0.91

1.17

0.98

 cpt1a

0.91

0.71

1.55

1.20

 PPARɣ

0.04***

0.12*

1.11

3.19

  1. Column 2 and 3 represent fold induction in PEMs relative to BMDMs grown in either 0 μg/mL oxLDL (macrophage, Mø) or 25 μg/mL oxLDL (foam cells, Fc) for 24 hours. The latter two columns represent the fold induction of gene expression in response to oxLDL stimulation in either BMDMs (column 4) or PEMs (column 5). n = 3 mice for BMDMs and 3 pools of 4–5 mice for PEMs. Two way ANOVA with Tukey’s post-test was applied. *p < 0.5, **p < 0.01, ***p < 0.001, ****p < 0.0001. ND: not detected. Italic >2 fold Bold <0.5 fold blank = no change. Abbreviations: Arginase 1 (Arg1), Chitinase 3-like 1 (Chi3l1), Suppressor of cytokine signaling 2 (Socs2), Chemokine (C-C motif) ligand 17 (CCL17), Mannose receptor, C type 1 (CD206), C-type lectin domain family 10, member A (MGL), Resistin like alpha (Retnla), Interleukin 10 (IL10), Macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), Interleukin 4 (IL4), CD163 antigen (CD163), Interleukin 12a (IL12a), Interleukin 6 (IL6), Prostaglandin-endoperoxide synthase 2 (Ptgs2), Tumor necrosis factor (TNF), Chemokine (C-C motif) ligand 22 (CCL22), Integrin alpha X (CD11c), Interferon beta 1 (Ifnß1), Nitric oxide synthase 2 (Nos2), Chemokine (C-X-C motif) ligand 10 (CXCL10), Interferon gamma (Ifnɣ), Macrophage scavenger receptor 1 (MSR1), Oxidized low density lipoprotein (lectin-like) receptor 1 (LOX1), Sterol Regulatory Element-Binding Protein 2 (SREBP2), Acyl-CoA synthetase long-chain family member 1 (ACSL1), ATP-binding cassette, sub-family G, member 1 (Abcg1), Diacylglycerol O-acyltransferase 2 (DGAT2), Scavenger receptor class B, member 1 (SRB1), CD36 antigen (CD36), Sterol Regulatory Element-Binding Protein 1 (SREBP1), ATP-binding cassette, sub-family A, member 1 (Abca1), Diacylglycerol O-acyltransferase 1 (DGAT1), Carnitine palmitoyltransferase 1a, liver (cpt1a), Peroxisome proliferator-activated receptor gamma (PPARγ).
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