Figure 4

Ka/Ks values calculated using full-length, PLAT and lipoxygenase domains from homologous genes.
PAL2NAL was used to convert amino acid sequences into the corresponding nucleotide sequences. PAML 4.0 was used to calculate the nonsynonymous/synonymous substitution (Ka/Ks) ratio. Ka/Ks values of 1, >1 and <1 indicated neutral, positive, and purifying selection, respectively.