Figure 6
CaV1.2e21+22 channels enhance ubiquitination of L-type CaV1.2 channels, but not T-type CaV3.1 channels.
CaV1.2e21+22 channels were co-transfected at a ratio of 1:1 with HA-CaV1.2e22, CaV3.1 or the chimeric CaV3.1GCGGG channels in HEK293 cells with or without MG132 treatment. Anti-HA and anti-CaV3.1 were used to pull down the protein complexes from cell lysates for ubiquitnation analysis of HA-CaV1.2e22 (A,B), wild-type CaV3.1 channels (C,D) or chimeric CaV3.1GCGGG channels (E,F) with or without MG132 treatment (n = 3). e22, HA-CaV1.2e22 channel. e21 + 22, CaV1.2e21+22 channel. Ub-CaV1.2, ubiquitinated CaV1.2 channels. Data were shown as mean ± SEM, ns, non-significant, *p < 0.05, #p < 0.01, 1-way ANOVA with post hoc Bonferroni’s test was performed for multiple comparisons.
