Figure 7
CaV1.2e21+22 channels compete for β2a subunit with L-type CaV1.2 channels, but not with T-type CaV3.1 channels, in a dose-dependent manner.
CaV1.2e21+22 channels were co-transfected at indicated ratios with HA-CaV1.2e22, CaV3.1 or chimeric CaV3.1GCGGG channels in HEK 293 cells treated with MG132. Anti-HA and anti-CaV3.1 were used for co-immunoprecipitation. Effects of CaV1.2e21+22 channels on the binding of β2a subunits to HA-CaV1.2e22 (A,B), CaV3.1 channels (C) or chimeric CaV3.1GCGGG channels (E) were analyzed by Western blotting (n = 3). e22, wild-type HA-CaV1.2e22 channel. e21 + 22, aberrant CaV1.2e21+22 channel. Data were shown as mean ± SEM, ns, non-significant, *p < 0.05, #p < 0.01, 1-way ANOVA with post hoc Bonferroni’s test was performed for multiple comparisons.
