Figure 3
Transcription profiles of PsRan in different Pst infection stages by qRT-PCR.
Pst development in wheat can be divided into three major stages, penetration stage (gray), parasitic/biotrophic stage (yellow), and sporulation stage (red). U, urediniospores; GU, in vitro germinated urediniospores; 18 h–216 h, wheat leaves infected with Pst at 18–216 hpi. Relative transcript levels were calculated by the comparative 2−ΔΔCT method and values are expressed relative to an endogenous Pst reference gene EF1. Results are composed of the means ± standard errors of three biological replications (each done in triplicates). Asterisks indicate a significant difference (P < 0.05) verus uredinospores using Student’s t test.
