Figure 4
HIGS of PsRan did not alter Pst virulence phenotype.
(a) Two specific sequence regions for HIGS of PsRan. (b) Phenotypes of the fourth leaves of BSMV:00- (control), BSMV:PsRan-1as-, and BSMV:PsRan-2as-inoculated wheat plants at 14 dpi with Pst. (c) Quantification of uredinial density in BSMV:00-, BSMV:PsRan-1as-, and BSMV:PsRan-2as-inoculated wheat plants 14 dpi with Pst. Values represent mean ± standard errors of three biological replications (each done in triplicates). (d) Relative transcript levels of PsRan in BSMV:00-, BSMV:PsRan-1as-, and BSMV:PsRan-2as-inoculated wheat plants at 18 hpi, 24 hpi, 48 hpi, and 14 dpi with Pst. Values are expressed relative to endogenous Pst reference gene EF1, with the empty vector (BSMV:00) set at 1. Values represent mean ± standard errors of three biological replications (each done in triplicates). Differences were assessed using Student’s t-tests and asterisks indicate P < 0.05.
