Figure 2

Impaired AKI repair in AIM felinised mice.

(a) Schema of the transgene. (b) Immunostaining in a non-reducing condition for feline AIM involving sera from AIM felinised mice (n = 3). AIM⁻ mouse serum and 3-SRCR feline rAIM (10 ng) were loaded as a negative and a positive control, respectively. IgM-bound and IgA-bound transgenic feline AIM are indicated. (c) The Cre levels on the indicated days after IR. Open circles, wild-type mice (n = 7); closed squares, AIM felinised mice (n = 6). Sham control mice (n = 3 for each) were also analysed. Grey circles, sham wild-type mice; grey squares, sham AIM felinised mice. Male mice at 8–10 weeks of age were used. ***P < 0.001. (d) Survival of wild-type and AIM felinised mice after IR (Kaplan-Meier method). P: statistical significance between each type of mouse was calculated by using the log-rank test. Solid line, wild-type mice (n = 7); dotted line, AIM felinised mice (n = 6). (e) Representative PAS-stained histologic photomicrographs of the kidney from wild-type and AIM felinised mice (n = 3 for each group) on day 3 after IR. Photomicrographs of the corticomedullary junction area are presented. Red arrows: brush border; black arrows: intraluminal debris. Scale bar: 50 μm. (f) The ATN score in the kidney of IR-treated wild-type mice and AIM felinised mice on day 3 after IR. n = 3 each.