Figure 4
Histopathology of tumors, lungs and livers of tumor-bearing mice after actein treatments.
(A) Endothelial cells in the tumor sections were assessed using Factor VIII and CD34 immunohistochemical analysis. Representative photomicrographs of magnification 40X showing the endothelial cells stained with anti-Factor VIII or anti-CD34 antibodies in brown. Quantification of Factor-VIII or CD34 positive-stained cells in tumor sections was conducted in a blinded manner. Results were expressed in box and whisker plots of 12 mice each group. (B) The paraffin-embedded sections of the lungs and livers were photographed and used to measure metastatic loci area and total lung or liver area. The histograms showed the tumor burden in lungs and livers according to the tumor area as a percentage of whole lung or liver area per group. Representative H&E-stained sections of lungs and livers from different groups with arrows showing the metastatic loci. Results were expressed in box and whisker plots of 12 mice each group. Differences among the treated and vehicle-treated control groups were determined by one-way ANOVA with Tukey’s post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 as compared to control group.
