Figure 2
Molecular weight and glycosylation analysis.
(A) SEC of marker proteins (1, 75 kDa conalbumin, 67 kDa bovine serum albumin, 45 kDa egg albumins, 29 kDa carbonic anhydrase, and 14.9 kDa lysozyme), native MdP2′GT (2, calculated MW 70.7 kDa), native (3, calculated MW 65.3 kDa) and denatured (4, calculated MW 35.2 kDa and 24.9 kDa) deglycosylated MdP2′GT with PNGase F, and native (5, calculated MW 65 kDa) and denatured (6, calculated MW 24.5 kDa) deglycosylated MdP2′GT with Endo H. (B) The calibration curve of the marker proteins. (C) SDS-PAGE analysis of (1) native MdP2′GT, (2) native and (3) denatured deglycosylated MdP2′GT with PNGase F, (4) 1 U PNGase F, (5) native and (6) denatured deglycosylation MdP2′GT with Endo H, and (7)10 U of Endo H. The specific activity of the native and native deglycosylated MdP2′GT under optimal conditions are indicated near the respective lanes. (D) MALDI-TOF spectra of (1)the native, (2)the native deglycosylated MdP2′GT with PNGase F or Endo H, and denatured deglycosylated MdP2′GT with (3)PNGase F or (4)Endo H.
