Table 2 Effects of various reagents on purified MdP2′GT activity.

From: Purification and characterization of a novel phloretin-2′-O-glycosyltransferase favoring phloridzin biosynthesis

Addition

Concentration/(mM)

Relative activity/(100%)

IC50/(mM)

None

—

100

—

NaCl

500

100 ± 1.00

ND

KCl

5

99.58 ± 2.31

ND

MgCl2

2

130.35 ± 3.53

Activator

NiCl2

5

98.26 ± 2.08

ND

CoCl2

5

0.00 ± 0.00

0.05 ± 0.01

CuCl2

5

0.00 ± 0.00

0.01 ± 0.01

CaCl2

5

54.21 ± 1.05

5.50 ± 0.12

BaCl2

5

99.03 ± 2.54

ND

MnSO4

5

47.41 ± 1.13

4.50 ± 0.11

FeSO4

5

119.43 ± 3.61

Activator

AlCl3

5

67.81 ± 1.03

8.00 ± 0.50

FeCl3

5

60.78 ± 1.01

6.50 ± 0.23

EDTA

5

99.16 ± 2.21

ND

Glycerol

686

98.13 ± 2.15

ND

AEBSF

0.5

99.51 ± 2.36

ND

β-mercaptoethanol

5

99.02 ± 1.47

ND

Dithiothreitol

5

99.15 ± 1.43

ND

Methanol

1236

99.23 ± 1.41

ND

Dimethyl sulfoxide

704

99.54 ± 1.40

ND

p-hydroxymercuribenzoate

1

18.45 ± 1.02

0.24 ± 0.07

N-Ethylmaleimide

1

20.53 ± 1.08

0.28 ± 0.01

UDP

5

0 ± 0.00

0.10 ± 0.01

UTP

5

3.05 ± 0.26

2.5 ± 0.10

UMP

5

38.25 ± 1.07

4.00 ± 0.13

Uridine

5

98.17 ± 2.12

ND

Trilobatin

5

21.70 ± 1.15

3.00 ± 0.21

3- hydroxyphloridzin

5

15.65 ± 0.58

2.50 ± 0.20

  1. Relative activities were calculated by comparison to a control in which none is added.
  2. No effect of addition on MdP2′GT activity was defined as relative activities ≥95%.
  3. ND: no effects detected at selected concentrations.
  4. Activator: additions increased MdP2′GT activity at selected concentrations.
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