Figure 1 | Scientific Reports

Figure 1

From: A Novel Pathway for Metabolism of the Cardiovascular Risk Factor Homoarginine by alanine:glyoxylate aminotransferase 2

Figure 1

Purification of human AGXT2.

Recombinant human AGXT2 was purified from tissues of transgenic mice with ubiquitous overexpression of human AGXT2 fused with a FLAG epitope at the C-terminus, by using an anti-FLAG affinity column. The identity of the protein was confirmed with both anti-FLAG and anti-AGXT2 antibodies. (A) Analysis of the purification steps by SDS-PAGE followed by staining with Coomassie blue. 1 – crude kidney homogenate, 2 – sample load, 3 – flow through, 4 – wash, 5 – elution. (B) Western Blot of the samples in (A) stained with the anti-FLAG antibody: 1 – crude kidney homogenate, 2 – sample load, 3 – flow through, 4 – wash, 5 – elution. (C) Western Blot of the elution fraction (corresponds to the lane 5 from the panels A and B) stained with the anti-AGXT2 antibody.

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