Table 1 Steady-state kinetic parameters of recombinant AGXT2 for the L-alanine/glyoxylate and L-homoarginine/pyruvate pairs.

From: A Novel Pathway for Metabolism of the Cardiovascular Risk Factor Homoarginine by alanine:glyoxylate aminotransferase 2

Substrate

Cosubstrate

Km L-ala (mM)

Km L-HA (mM)

kcat (min−1)

kcat/Km (min−1/mM−1)

L-alanine

Glyoxylate

25 ± 3

 

8.5 ± 0.5

0.34 ± 0.04

L-homoarginine

Pyruvate

 

24 ± 3

1.13 ± 0.04

0.047 ± 0.006

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