Figure 9 | Scientific Reports

Figure 9

From: Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions

Figure 9

Humoral immune response against the B. microti secretome.

An antigen down protein array consisting of 198 selected B. microti polypeptides corresponding to 174 unique proteins and screened it using pre-immune as well as immune sera collected from wild type Swiss Webster mice at days 4, 8, 12, and 16 following inoculation with B. microti LabS1 strain. Heatmaps for all 198 polypeptides were generated with the IgG (A) and IgM (B) intensity data. The color scale goes from white > grey > red representing low > mid > high serum reactivity to the spots. Each row is a feature/protein on the microarray and each column is a sample. A zoomed in view for the top 20 reactive antigens with row labels was also generated (C,D). This same data was used to create a line graph for the top 20 reactive antigens to show binding kinetics for IgG (E) and IgM (F) antibodies more clearly.

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