Figure 5
Deletion of cydAB impairs survival in a mouse UTI model.
A minimum of eight C57BL/6 mice were transurethrally inoculated with ~5 × 108 CFU of each strain. After 48 h, bladder homogenate samples were plated on MacConkey agar in triplicate for determination of bacterial loads. After 2 days, homogenated bladder samples were processed for bacterial loads by viable CFU counts performed in triplicate. Data are Log of the fitness index, which is defined as: Fitness index = (ratio of mutant:wild type after 2 days)/(initial mutant:wild type ratio). A minimum of 8 mice were included in each strain group. Equality of group medians was tested using the Wilcoxon matched pairs signed rank test.
