Figure 2

Generation of polyclonal antibodies against TgCdk7, TgCyclinH and TgMat1 which specifically identifies the native protein in the parasite.

(A) Polyclonal antibodies raised against TgCdk7 were specific as western blot analysis with immune sera recognized the band of expected size (~46 kDa) in the native parasite lysate. The antibodies also recognized GST-TgCdk7 recombinant protein (~72 kDa). (B) Similarly polyclonal antibodies recognized TgCyclinH native (~65 kDa) and recombinant (GST-tag) proteins (~92 kDa). (C) TgMat1 antibody identified both native and recombinant (His-tag) proteins at the predicted size (~32 kDa). All the three antibodies raised did not display cross-reactivity with HFF proteins.