Figure 6
From: Cdk7 mediates RPB1-driven mRNA synthesis in Toxoplasma gondii
Phosphorylation of TgRPB1-CTD abrogates promoter DNA binding.
Unphosphorylated CTD binds to DNA (lane 2). Binding was substantially decreased in a concentration dependent manner when CTD is phosphorylated by activated kinase Tg-Cdk7/CyclinH/Mat1. The DNA binding remained unaltered in the presence of inhibitor BS-181 (lane 3). Kinase reaction control showing CTD phosphorylation in the presence of activated kinase is shown in lower panel.
