Figure 10
Gliotoxin detection via RP-HPLC and LC-MS in ATCC26933 and ΔegtA26933.
(a) RP-HPLC analysis of organic extracts from the supernatants of 72 h cultures of ATCC26933 and ΔegtA26933. Gliotoxin is present in all chromatograms for both samples at 14.9 min. (b) Comparison of gliotoxin peak area from RP-HPLC analysis for ATCC26933 and ΔegtA26933 performed in triplicate. The peak area was found to be significantly (P = 0.0003) lowered in ΔegtA26933. (c) Extracted Ion Chromatographs (m/z: 327) following LC-MS analysis of organic extracts of supernatants from ATCC26933 and ΔegtA26933, in addition to a gliotoxin standard. A peak at 6.3 min was confirmed to be gliotoxin. (d) Signature ion breakdown corresponding to gliotoxin.
