Figure 7
GSH detection via LC-MS in ATCC26933, ΔegtA26933 and egtAC26933.
(a) Extracted Ion Chromatographs (m/z: 695) following LC-MS analysis of TCA precipitated 5′-IAF-labelled mycelial extracts from ATCC26933, ΔegtA26933 and egtAC26933, in addition to a GSH standard. A peak at 5.9 min was confirmed to be GSH. (b) Peak height data from LC-MS analysis comparing GSH levels in ATCC26933, ΔegtA26933 and egtAC26933. GSH levels are significantly (P = 0.0016) increased in ΔegtA26933 compared to the wild-type and complemented samples. (c) Signature Ion breakdown corresponding to 5-IAF labelled GSH.
