Figure 1
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
Autoreactive CD8+ T-cells expressing low affinity TCRs are highly CD8-dependent.
6 × 104 C1R-A*0201 (WT MHCI/CD8) or C1R-A*0201 D227K/T228A (Null MHCI/CD8) target cells were pulsed with cognate peptide at the indicated concentrations in duplicate for 1 hour at 37 °C. 3 × 104 clonal 1E6 (A,B), ALF8 (C,D) or MCNLV (E,F) CD8+ T-cells were then added and the plates were incubated for 4 hours at 37 °C. Supernatants were assayed for IFN-γ and MIP-1β by ELISA. The mean ± SD of two replicate assays is shown. Data is representative of three independent experiments.
