Figure 2
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
CD8 has a profound effect on the number of peptides that can be recognised by the diabetogenic CD8+ T-cell clone 1E6.
6 × 104 C1R-A*0201 (WT MHCI/CD8) or C1R-A*0201 D227K/T228A (Null MHCI/CD8) target cells were pulsed in duplicate with each mixture from a 10mer combinatorial peptide library (100 μM) for 2 hours at 37 °C. 3 × 104 clonal 1E6 CD8+ T-cells were then added and the plates were incubated overnight at 37 °C. Supernatants were assayed for MIP-1β by ELISA. The primary screens with wildtype (WT) targets were reported previously40; the primary screens with D227K/T228A (Null) targets are shown in Supplementary Fig. S2. A box plot summary is presented with the index sequence at the bottom. Data is representative of two independent experiments.
