Figure 3
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
Anti-CD8 antibodies can block pMHCI tetramer binding at low TCR/pMHCI affinities.
5 × 104 clonal 1E6 (A,B) or ALF8 (C,D) CD8+ T-cells were pre-treated with or without the indicated anti-human CD8 antibodies at various concentrations for 20 minutes at 4 °C and then stained with 25 μg/ml of cognate pMHCI tetramer for 15 minutes at 37 °C. The column graphs depict percent maximal tetramer staining (A,C) or percent inhibition of tetramer staining (B,D). Data is representative of three independent experiments.
