Figure 4
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
DK25 blocks the activation of CD8+ T-cells at low TCR/pMHCI affinities.
2 × 103 51Cr-labeled C1R-A*0201 target cells were peptide-pulsed with either ALWGPDPAAA (A,B) or RQWGPDPAAV (A,C) at the indicated concentrations for 1 hour at 37 °C. 1 × 104 clonal 1E6 CD8+ T-cells were then added with or without the anti-human CD8 antibody DK25 at a final concentration of 0.5 or 1 μg/ml. Cytotoxicity was determined after 4 hours as described in the Materials and Methods. The mean ± SD of three replicate assays is shown. Data is representative of three independent experiments.
