Figure 5
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
DK25 selectively blocks autoreactive CD8+ T-cell activation.
6 × 104 C1R-A*0201 target cells were peptide-pulsed with ALWGPDPAAA (A,B), GILGFVFTL (D) or NLVPMVATV (F) at the indicated concentrations for 1 hour at 37 °C. In parallel, 6 × 104 K562-A*2402 or C1R-B*3508 target cells were peptide-pulsed similarly with LWMRLLPLL (C) or LPEPLPQGQLTAY (E), respectively. 3 × 104 clonal 1E6 (A), 3F2 (B), 4C6 (C), ALF3 (D), SB27 (E) or MCNLV (F) CD8+ T-cells pre-treated for 30 minutes at 37 °C with the anti-human CD8 antibody DK25 at the indicated concentrations were then added and the plates were incubated for 4 hours at 37 °C. Supernatants were assayed for MIP-1β by ELISA. The mean ± SD of two replicate assays is shown. Data is representative of two independent experiments.
