Figure 6
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
DK25 selectively blocks autoreactive CD8+ T-cell killing.
2 × 103 51Cr-labeled C1R-A*0201 (A,B,D,F), K562-A*2402 (C) or C1R-B*3508 (E) target cells were peptide-pulsed with ALWGPDPAAA (A,B), LWMRLLPLL (C), GILGFVFTL (D), LPEPLPQGQLTAY (E) or NLVPMVATV (F) at the indicated concentrations for 1 hour at 37 °C. 1 × 104 clonal 1E6 (A), 3F2 (B), 4C6 (C), ALF3 (D), SB27 (E) or MCNLV (F) CD8+ T-cells were then added with or without the anti-human CD8 antibody DK25 at a final concentration of 0.25, 0.5 or 1 μg/ml. Cytotoxicity was determined after 4 hours as described in the Materials and Methods. The mean ± SD of three replicate assays is shown and is representative of two independent experiments.
