Figure 9
From: Targeted suppression of autoreactive CD8+ T-cell activation using blocking anti-CD8 antibodies
CT-CD8a blocks the activation of autoreactive murine CD8+ T-cells.
6 × 104 P815 murine mastocytoma cells were peptide-pulsed with LYLVCGERG at the indicated concentrations for 1 hour at 37 °C. 3 × 104 splenic G9C8 TCR-transgenic CD8+ T-cells pre-treated for 30 minutes at 37 °C with either the anti-mouse CD8 antibody YTS105.18 (A) or the anti-mouse CD8 antibody CT-CD8a (B) at the indicated concentrations were then added and the plates were incubated overnight at 37 °C. Supernatants were assayed for MIP-1β by ELISA. The mean ± SD of two replicate assays is shown and is representative of two independent experiments.
