Figure 5
From: Longer latency of sensory response to intravenous odor injection predicts olfactory neural disorder
Orthonasal airflow blockage reduces response duration but has no effect on onset latency.
(a) Schematic of orthonasal airflow blockade. Upper, a silicon tube is inserted into the rostral part of the unilateral nostril; Lower, silicon-tube insertion blocks orthonasal airflow. (b) Image of the OB dorsal surface before prosultiamine (PST) administration. Scale bar, 500 μm. (c) Intensity histograms of spH signals from the dorsal surface of the OB on the occluded and open sides. Blue arrow shows median signal intensity of the occluded side, magenta arrow shows that of the open side. (d) Comparison of signal intensities between occluded (blue) and open sides (magenta). occl., occluded side. (e) A representative trace of the relative spH-signal intensity from the dorsal surface of the OB on the occluded side. The trace is represented as intensity of the occluded side compared with the open side. Note that nostril occlusion without PST administration did not induce significant signal-changes over a period of 600 s. (f) A representative photomicrograph of the OE stained with anti-OMP on the occluded side. Scale bar, 20 μm. (g) Comparison of the number of OMP-positive cells between occluded and open sides. (h) Comparison of peak PST-induced spH signals between occluded (blue) and open sides (magenta). (i) Images of the OB dorsal surface on the occluded (left) and open side (right). g1–g7 are individual glomeruli. Scale bar, 500 μm. (j) PST-induced spH signals from individual glomeruli on the occluded (left, g1–g7) and open sides (right, g1–g7). Open rectangle, onset latency; filled rectangle, duration. (k) Summary of onset latency and duration for each glomerulus shown in (h,i). Diamond, onset time; square, duration. (l,m) Comparison of onset latency (k) and duration (l) between occluded and open sides. There is no significant difference in the onset time between occluded and open sides, whereas the duration is significantly shorter on the occluded side. Mann-Whitney U-test (d,f,g,k,l). n.s., no significant difference, *p < 0.05, ***p < 0.001. All values are mean ± SD.
