Figure 2
From: Hepatic 3D spheroid models for the detection and study of compounds with cholestatic liability
Exposure to hepatotoxins in the presence of bile acids reveals a selective synergistic toxicity of compounds with known cholestatic liability and bile acids.
PHH and HepaRG spheroids were repeatedly exposed to a set of compounds with known cholestatic liability (bosentan, chlorpromazine and troglitazone) and compounds with non-cholestatic hepatotoxicity patterns (acetaminophen and tetracycline) in the presence or absence of a non-toxic BA mixture for 8 days (A) and 14 days (B). Cellular ATP content was measured as a marker for viability. Each data point is the mean ± SEM viability of n = 4–6 spheroids. Data are representative of 3 independent experiments.
