Figure 3
From: Hepatic 3D spheroid models for the detection and study of compounds with cholestatic liability
Cholestatic risk classification of the tested hepatotoxins based on the cholestatic index (CIx).
(A,B) Classification of the cholestatic risk of the tested compounds in PHH and HepaRG spheroids after 8 and 14 days of repeated exposure as determined by the cholestatic index (CIx). Three independent experiments for each compound are presented. Viability was assessed by measuring the cellular ATP content. (C) CIx values of a panel of hepatotoxins determined in multi-donor PHH spheroids after 14 days of repeated exposure. Viability was assessed by measuring albumin secretion, which has similar sensitivity to measuring the cellular ATP content in terms of determining the CIx values (Supplementary Fig. S2). For all figures: green: CIx ≥ 0.80; red: CIx ≤ 0.80.
