Figure 4
From: Hepatic 3D spheroid models for the detection and study of compounds with cholestatic liability
Mechanisms of chlorpromazine-induced cholestasis are recapitulated in PHH and HepaRG spheroids.
PHH and HepaRG spheroids were repeatedly exposed to the model cholestatic compound chlorpromazine (CPZ) for 8 days. (A) BA accumulation was assessed by using the fluorescently labelled taurocholic acid derivative tauro-nor-THCA-24-DBD. Note that 10 μM CPZ was toxic to the HepaRG spheroids. (B) Expression of ABCB11, encoding BSEP, was analysed by RT-qPCR and normalized to the expression of the housekeeping gene GAPDH. Data represent the means ± SEM of 3 independent experiments. **p < 0.01. (C) F-actin cytoskeleton integrity was visualized by Phalloidin staining. All scale bars = 100 μm.
