Figure 5
From: Hepatic 3D spheroid models for the detection and study of compounds with cholestatic liability
Oxidative stress and modulation of death receptor signalling underlie the synergistic toxicity of chlorpromazine and bile acids.
PHH and HepaRG spheroids were repeatedly exposed to chlorpromazine (CPZ) or acetaminophen (APAP) in the presence or absence of a non-toxic BA mixture. After 8 days, expression levels of the Nrf2 target gene SRXN1 (A), NFE2L2 (B) and DR5 (C) were evaluated by RT-qPCR and normalized to the expression of the housekeeping gene GAPDH. Data represent the means ± SEM of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
