Figure 2

Puerarin inhibited Kir2.1 and Kir2.3 channels in a dosage dependent manner.

(a) Puerarin at the concentrations of 10 μM, 100 μM, 10 mM, 50 mM, 100 mM, 200 mM significantly inhibited Kir2.1 currents illustrated by the ramp voltage protocol, respectively. This effect could be partly reversed by simple compound wash-off. (b) Concentration dependent inhibition by puerarin upon Kir2.1. The maximal inhibitory effect was (53.11 ± 1.84) % and saturation dosage was 50 mM. Dosage curve was fitted with non-linear Hill. IC₅₀ = 1.27 mM, Hill coefficient is 0.990. n = 5 each point. (c) Puerarin at the concentrations of 10 μM, 100 μM, 1 mM, 10 mM, 50 mM, 100 mM, 200 mM significantly inhibited Kir2.3 currents illustrated by the ramp voltage protocol, respectively. This effect could be partly reversed by simple compound wash-off. (d) Concentration dependent inhibition by puerarin upon Kir2.3. Dosage curve was fitted with non-linear Hill. IC₅₀ = 129.4 mM, Hill coefficient is 0.984. n = 5 each point.