Figure 4
From: The Effects of Puerarin on Rat Ventricular Myocytes and the Potential Mechanism
Puerarin inhibited IKs channels in a dosage dependent manner.
(a) Puerarin at the concentrations of 10 μM, 100 μM, 1 mM and10 mM significantly inhibited IKs currents illustrated by the protocol of voltage steps, respectively. (b) I-V curves summarized inhibitory effects of puerarin at concentrations of 10 μM, 100 μM, 1 mM, 10 mM and recovery after compound wash off. Different concentrations of puerarin decreased the IKs from (33.8 ± 3.2) pA/pF to (25.6 ± 2.4) pA/pF, (15.7 ± 1.9) pA/pF, (6.0 ± 1.3) pA/pF, (5.4 ± 1.7) pA/pF, and decreased the IKs-tail current from (23.5 ± 1.2) pA/pF to (17.8 ± 1.5) pA/pF, (9.7 ± 1.1) pA/pF, (6.4 ± 1.2) pA/pF, (5.2 ± 1.3) pA/pF at 40 mV, respectively. (c) Concentration dependent inhibition by puerarin upon IKs at the peak currents and tail currents. Dosage curve was fitted with non-linear Hill. IC50 = 55.63 μM at the peak currents and IC50 = 59.75 μM at the tail currents, Hill coefficient is 0.989. n = 6 each point.
