Figure 7
Lack of s36 chorionic protein from regionally specialized follicle-cell subpopulations does not harm micropylar or polar integrity, but causes an age-dependent sporadic derailment of dorsal-appendages’ patterning.
(A,D) Confocal laser scanning microscopy (CLSM) images of representative stage-10 control follicles specifically expressing the eGFP protein either in the border-cell cluster (c522 > eGFP) (A) or in the (anterior and posterior) polar-cell clusters (109c1 > eGFP) (D). (B,C) Scanning electron microscopy (SEM) images of follicles being specifically targeted for the s36 protein in border cells (c522 > s36_RNAi). (E,F) SEM images of follicles being specifically targeted for the s36 protein in (anterior and posterior) polar cells (109c1 > s36_RNAi). (E) Anterior end. (F) Posterior end. (G) CLSM image of a representative stage-14 control follicle specifically expressing the eGFP protein in follicle cells of dorsal appendages (109-28 > eGFP). (H,I) SEM images of follicles being specifically targeted for the s36 protein in follicle cells of dorsal appendages (109-28 > s36_RNAi). (J) Bar-chart presenting the percentage (%) of follicles with a sporadically acquired and fly age-driven (0–29 and 30–60 days) pathology of dorsal appendages being produced by 109-28 > s36_RNAi but not 109-28-GAL4/+ (control) fly strains. (K,L) Light microscopy images of 109-28 > s36_RNAi follicles being sporadically generated by aged flies. Dysmorphic follicles are characterized by the development of four (K) or three (L), instead of two (e.g. Fig. 7H), dorsal appendages. Scale Bars: 50 μm in A,D,G,H,K,L; 10 μm in B,C,E,F,I.
