Figure 6

Prokaryotic expression of cellulases.
(A) Prokaryotic expression of Thph1 and Thph2. Lane M molecular marker; Lane 1, ΔThph1 supernatant; Lane 2, ΔThph2 supernatant; Lane 3, Ni-purified Thph1 protein; Lane 4, Ni-purified Thph2 protein. (B) Detection of the target protein by western blot analysis (CK, 1% carboxymethylcellulose; WT, wild-type strain; 1, mutants containing Thph1 + His tag; 2, mutants containing Thph2 + His tag).