Figure 1
From: Problems and Solutions in Click Chemistry Applied to Drug Probes
Mass spectrometry analysis of cysteine and alkyne and co-localization of FITC-alkyne and TRITC-azide.
(a) The interference of cysteine in the probe fishing experiment is shown. (b) Treatment with hydrogen peroxide prevents cysteine interference. (c) Schematic diagrams depicting the oxidation treatment of experimental cells with hydrogen peroxide. (d) The results of the conventional co-localization experiment indicating low co-localization efficiency. (e) Co-localization after oxidation treatment with hydrogen peroxide. The degree of co-localization of FITC-alkyne and TRITC-azide is significantly increased. (f) Co-localization of imatinib and CD117; the co-localization rate increased after treatment with hydrogen peroxide.
