Figure 1
Schematic diagram of PvGAMA, fragments of PvGAMA for recombinant protein expression, erythrocyte binding assay and processing of PvGAMA.
(a) Schematic diagram of PvGAMA. The PvGAMA protein comprises 771 amino acids with a calculated molecular mass of 82.5 kDa. Two regions of PvGAMA, PvGAMA-Ecto (Ecto; amino acid (aa) position 22–771) and -Tr1 (Tr1; aa 22-590), were designed for protein expression, and 7 fragments (F1, aa 22–344; F2, aa 345–589; F3, aa 590–771; F4, aa 22–589; F5, aa 345–771; F6, aa 408–746 and F7, aa 408–589) were designed for the erythrocyte binding assay. Indicated are the predicted signal peptide (SP; aa position 1–21) and glycosylphosphatidylinositol-anchor signal (GPI; aa 747–771). PvGAMA-Ecto and -Tr1 were used to raise specific antisera. (b) Processing of PvGAMA was predicted from western blot results of the parasite lysate probed with anti-PvGAMA-Ecto sera (Fig. 2c). aa, amino acid; kDa, kilodalton; SP, signal peptide; GPI, glycophosphatidylinositol-anchor signal; Cys, Cysteine; Asn, Asparagine.
