Figure 3
From: Harnessing the natural inhibitory domain to control TNFα Converting Enzyme (TACE) activity in vivo
Treatment with TPD in collagen induced arthritis in vivo mice model and in human synovial cells.
Collagen induced arthritis (CIA) in DBA/1 mice by immunization with type II collagen and intravenous injection of TPD 1 and 3 mg/Kg, 1 day after type II collagen boost and stopped after 10 days of daily injection. Macroscopic clinical score (a), representative photo of the inflamed joints (d), histopathologic analysis performed in hematoxylin/eosin-stained sections of joints (c) and serum from naïve, PBS control arthritic mice and TPD treated were analysed for anti-collagen antibodies (d). *P < 0.05, n = 10 (Mean ± SEM Student’s t test). (e) TPD incubation with synovial fluids derived from RA patient analyzed with human TNFα ELISA. *P < 0.05, n = 3 (Mean ± SD Student’s t test).
