Figure 1

CRISTAL sample preparation and workflow of the correlative study.

(a) Sample preparation steps of a rat lung lobe with the CRISTAL method. The accessory lung lobe of the rat is shown after fixation and after the resin-based clearing. (b) Workflow of the correlative study. First, the CRISTAL sample was screened by using SLOT. Based on the identified ROI, further investigations like MPM, histology or TEM were carried out. The CRISTAL block was adapted to the requirements of the various corresponding imaging techniques and sectioned for histology and immunofluorescence as well as TEM.