Table 1 Relative quantities of candidate reference miRNAs in gnome-wild serum profile.

From: Identification of reference genes for circulating microRNA analysis in colorectal cancer

miRNA

Ct (Healthy) (Mean ± SE)

Ct (Patient) (Mean ± SE)

Fold change

P value

hsa-miR-101-3p

27.70 ± 0.02

28.09 ± 0.06

−1.309

0.024

hsa-miR-16-5p

25.88 ± 0.02

25.64 ± 0.04

1.180

0.026

hsa-miR-93-5p

30.31 ± 0.04

31.22 ± 0.01

−1.873

0.002

hsa-miR-320d

28.76 ± 0.01

28.85 ± 0.01

−1.069

0.051

hsa-miR-25-3p

28.24 ± 0.01

28.34 ± 0.03

−1.067

0.069

hsa-miR-92b-3p

31.91 ± 0.20

31.96 ± 0.20

−1.030

0.895

hsa-miR-106b-5p

28.69 ± 0.04

28.65 ± 0.05

1.028

0.619

hsa-miR-10b-5p

32.05 ± 0.08

31.99 ± 0.01

1.045

0.495

hsa-miR-107

31.16 ± 0.03

31.07 ± 0.07

1.061

0.362

hsa-miR-574-5p

24.85 ± 0.22

24.77 ± 0.09

1.062

0.747

  1. Hsa-miR-101-3p, hsa-miR-16-5p and hsa-miR-93-5p have been reported as normalizers in literatures; seven additional putative miRNAs (hsa-miR-320d, hsa-miR-25-3p, hsa-miR-92b-3p, hsa-miR-106b-5p, hsa-miR-10b-5p, hsa-miR-107 and hsa-miR-574-5p) were extracted with standards: fold change < 1.1 and P value > 0.05. Data of ten miRNA was from profiling data set in this study to make sure consistent results. All the Ct value was less than 32.
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