Figure 1 | Scientific Reports

Figure 1

From: Molecular characterization of host-parasite cell signalling in Schistosoma mansoni during early development

Figure 1

PKC and ERK activation during S. mansoni early somule development in vitro.

(a) Detection of phosphorylated (activated) PKCs (p-PKC) and ERKs (p-ERK) in somules during 96 h culture by western blotting using anti-phospho PKC (Ser660)/(Thr410) or anti-phospho p44/42MAPK (Thr202/Tyr204) (ERK1/2) antibodies. Anti-actin antibodies were used to monitor differences in total protein loading between lanes. (b) In situ localization of activated kinases (green) within somules at 3, 16 and 96 h using anti-phospho PKC (Ser660)/(Thr410) or anti-phospho p44/42MAPK (Thr202/Tyr204) (ERK1/2) primary and AlexaFluor 488 secondary antibodies and confocal microscopy. F-actin is stained with rhodamine phalloidin (red). Representative micrographs of somules are shown as z-axis maximum projections. Bar = 25 μm.

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