Figure 1
From: A rapid co-culture stamping device for studying intercellular communication
Microfluidic stamp structure and patterning.
(A) Stamping procedure: (a) The stamp is placed on a flat substrate such as a glass or a tissue-culture dish. (b) Cell suspension is injected to the first inlet using a standard lab pipettor. The cell suspension fills the channels while allowing residual air to escape through the air vent. (c) In a similar manner, a second cell suspension is injected from a second inlet. (d) Following cell sedimentation and on-chip incubation, the PDMS stamp is removed while leaving cells attached to the substrate. (B) Device channel visualization by showing image of the device injected with blue and orange food colours. Zoomed image shows the restriction and air vent that limit liquid flow but allow air to escape. Scale bar, 1 mm and 200 μm (C) Fibroblasts L929 patterned on a TC plate. Scale bar 300 μm (D) Fluorescent-conjugated protein patterning. Scale bar: 500 μm.
