Figure 3
From: Optogenetics design of mechanistically-based stimulation patterns for cardiac defibrillation
Optical manipulation of the cardiac conduction pathway.
Four patterns of optogenetic stimulation: single-point; single-barrier, triple-barrier, and whole LV, designed as reported by the blue traits on the fluorescence image (F0) of the heart. The green frame represents the AODs working-field on the sample. Corresponding frames of fluorescence signal (ΔF/F0) are reported next with the relative isochronal maps. For each isochronal map the scale has been individually adjusted to better highlight the activation times of every pattern. On the right, capture probability (Pcapt) for each ChR2 stimulation pattern based on pulse duration and laser power. Pcapt is defined as the percentage of all flashes given that successfully captured the ventricle. Different colors (blue, green, and red) depict different laser intensities. Note that the laser power is maintained constant for each color for every pattern (blue = 1.3 mW, green = 3.4 mW, and red = 4.9 mW), with the exception of the whole LV illumination, in which laser power has been increased four times. Data reported as mean ± SEM (standard error of the mean) from N = 3. Scale bar of 2 mm in yellow.
