Table 1 Sequences of primers used for PCR.
From: Osterix represses adipogenesis by negatively regulating PPARγ transcriptional activity
Gene | Primer sequence (5′→3′) | |
|---|---|---|
PCR | Osterix | (F) 5′-GGC ACA AAG AAG CCG TAC TC-3′ |
(R) 5′-GCC TTG TAC CAG GAG CCA TA-3′ | ||
C/EBPβ | (F) 5′-AGC CCC TAC CTG GAG CCG CT-3′ | |
(R) 5′-GCG CAG GGC GAA CGG GAA AC-3′ | ||
C/EBPα | (F) 5′-TGC TGG AGT TGA CCA GTG AC-3′ | |
(R) 5′-AAA CCA TCC TCT GGG TCT CC-3′ | ||
PPARγ | (F) 5′-ATC AGC TCT GTG GAC CTC TC-3′ | |
(R) 5′-ACC TGA TGG CAT TGT GAG AC-3′ | ||
GAPDH | (F) 5′-ACC ACA GTC CAT GCC ATC AC-3′ | |
(R) 5′-TCC ACC ACC CTG TTG CTG TA-3′ | ||
qPCR | Osterix | (F) 5′-AGC GAC CAC TTG AGC AAA CAT-3′ |
(R) 5′-GCG GCT GAT TGG CTT CTT CT-3′ | ||
PPARγ | (F) 5′-GCC CTT TGG TGA CTT TAT GGA-3′ | |
(R) 5′-GCA GCA GGT TGT CTT GGA TG-3′ | ||
aP2 | (F) 5′-GAA CCT GGA AGC TTG TC-3′ | |
(R) 5′-CGT GAC TTC CAC AAG AGT-3′ | ||
Adiponectin | (F) 5′-CCC AGG ACA TCC TGG CCA CAA-3′ | |
(R) 5′-CCT TCA GCT CCT GTC ATT CCA-3′ | ||
GluT4 | (F) 5′-GTA ACT TCA TTG TCG GCA TGG-3′ | |
(R) 5′-AGC TGA GAT CTG GTC AAA CG-3′ | ||
GluT1 | (F) 5′-TCA ACA CGG CCT TCA CTG-3′ | |
(R) 5′-CAC GAT GCT CAG ATA GGA CAT C-3′ | ||
GAPDH | (F) 5′-AGG TCG GTG TGA ACG GAT TTG-3′ | |
(R) 5′-GGG GTC GTT GAT GGC AAC A-3′ |
